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研究生教育

药理学

吴振永

姓 名 吴振永 性 别 职 称 研究员、博导
学 历 博士 电 话 传 真
电子邮件 wuzhenyong@simm.ac.cn 个人主页 主页链接 专家类别
职 务 课题组长、博导
通讯地址 烟台市牟平区滨海东路198号
个人简介
工作经历
     
     
     
     
研究方向

1. 利用纳米技术探索神经递质释放核心分子机制及其在重大脑疾病中致病机理。

2.通过人工分离突触囊泡研究兴奋性和抑制性神经递质释放失衡的分子机制。

3.可控生物纳米材料药物对外周及中枢神经系统的靶向递送。


科研项目
科研成果

1.原创开发了纳米盘与细胞融合技术。 阐明了突触囊泡蛋白VAMP2扩大囊泡融合孔的分子机理。

2. 运用DNA纳米技术对携带SNARE蛋白的人工囊泡进行亚分类。将囊泡的曲率推进到理论极限值,研究了其对生物膜融合的直接影响。

3.利用新型荧光分子探针进一步阐明了神经递质非同步释放分子机制, 探索了单个神经元神经递质不同发放模式的空间分布特点。


荣誉获奖
    代表论著

      1.Wu ZY, Dharan N, McDargh ZA, Thiyagarajan S, O'Shaughnessy B, Karatekin E. The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores. eLife. 2021.

      2.Yang Y#, Wu ZY#, Wang L, Zhou K, Xia K, Xiong Q, Liu L, Zhang Z, Chapman ER, Xiong Y, Melia TJ, Karatekin E, Gu H, Lin C. Sorting sub-150-nm liposomes of distinct sizes by DNA- brick-assisted centrifugation. Nature Chemistry. 2021 (# 共同 作者) (This article was featured by News and Reviews section of Nature Chemistry: https://www.nature.com/articles/s41557-021-00673-7).

      3.Courtney K, Vevea J, Li YQ, Wu ZY, Zhang Z, Chapman E. Synaptotagmin 1 oligomerization via the juxtamembrane linker regulates spontaneous and evoked neurotransmitter release. Proc Natl Acad Sci USA. 2021.

      4.Courtney K, Wu LX, Mandal T, Swift M, Zhang Z, Alaghemandi M, Wu ZY, Bradberry M, Deo C, Lavis L, Volkmann N, Hanein D, Cui Q, Bao H, Chapman E. The complexin C-terminal amphipathic helix stabilizes the fusion pore open state by sculpting membranes. Nat Struct Mol Biol, 2022.

      5.Wu ZY, Ma L, Nicholas AC, Zhu J, Zhang YL, Edwin R.C, Karatekin E. Polybasic patches in both C2 domains of Synaptotagmin-1 are required for evoked neurotransmitter release. J Neurosci. 2022.

      6.Dudzinski NR, Wu ZY*, Karatekin E*. A Nanodisc-Cell Fusion Assay with Single-Pore Sensitivity and Sub-millisecond Time Resolution. Methods Mol Biol. 2019. (*共同通讯作者).

      7.Ma L, Cai Y, Li Y, Jiao J, Wu ZY, O'Shaughnessy B, De Camilli P, Karatekin E, Zhang Y. Single-molecule force spectroscopy of protein-membrane interactions. eLife. 2017.

      8.Wu ZY, Thiyagarajan S, O'Shaughnessy B, Karatekin E. Regulation of Exocytotic Fusion Pores by SNARE Protein Transmembrane Domains. Frontiers in Molecular Neuroscience. 2017.

      9.Li Y, Li F, Bai B, Wu ZY, Hou X, Shen Y, Wang Y. Protein interacting with C‑kinase 1 modulates exocytosis and KATP conductance in pancreatic β cells. Mol Med Rep. 2017.

      10.Wu ZY, Bello OD, Thiyagarajan S, Auclair SM, Vennekate W, Krishnakumar SS, O'Shaughnessy B, Karatekin E. Dilation of fusion pores by crowding of SNARE proteins. eLife. 2017 (F1000 recommended: https://facultyopinions.com/prime/727446608; Score 18.7; on top of % 0.5 in Cell Biology).

      11.Jin YY, He MZ, Wu ZY, Huang K, Shen Y, Liang L, Mao JH. Dysregulation of calcium channels decreases para-secretion in pancreatic β-cells in rats born small for gestational age. Growth Factors. 2016.

      12.Wu ZY, Auclair SM, Bello O, Vennekate W, Dudzinski NR, Krishnakumar SS, Karatekin E. Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains. Scientific Reports. 2016 (F1000 recommended: Kavalali E: Faculty Opinions Recommendation of [Wu Z et al., Sci Rep 2016 6:27287]. In Faculty Opinions, 18 Sep 2017; 10.3410/f.726403621.793536784. https://facultyopinions.com/prime/726403621).

      13.Stratton BS, Warner JM, Wu ZY, Nikolaus J, Wei G, Wagnon E, Baddeley D, Karatekin E, O'Shaughnessy B. Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores. Biophys J. 2016.

      14.Zou N, Wu X, Jin YY, He MZ, Wang XX, Su LD, Rupnik M, Wu ZY*, Liang L*, Shen Y*. ATP regulates sodium channel kinetics in pancreatic islet beta cells. J Membr Biol. 2013 (* 共同通讯作者).

      15.Zhu J, Shao CY, Yang W, Zhang XM, Wu ZY, Zhou L, Wang XX, Li YH, Xia J, Luo JH, Shen Y. Chronic zinc exposure decreases the surface expression of NR2A-containing NMDA receptors in cultured hippocampal neurons. PLoS One. 2012.

      16.Wu ZY, Zhu LJ, Zou N, Bombek LK, Shao CY, Wang N, Wang XX, Liang L, Xia J, Rupnik M, Shen Y. AMPA receptors regulate exocytosis and insulin release in pancreatic β cells. Traffic. 2012.

      17.Wu ZY, Zou N, Shen Y. Glutamate receptors and gamma-aminobutyric acid receptors in pancreatic cells. Sheng Li Ke Xue Jin Zhan. 2011. 


    社会任职

    2013-2019 美国生物物理学会会员

    吴振永

    姓 名 吴振永 性 别 职 称 研究员、博导
    学 历 博士 电 话 传 真
    电子邮件 wuzhenyong@simm.ac.cn 个人主页 主页链接 专家类别
    职 务 课题组长、博导
    通讯地址 烟台市牟平区滨海东路198号